The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the UNIPROT ALIGN P12956. N P E G K V

Jan 1, 2017 Single-strand DNA-dependent helicase of the KU70/KU80 complex participates in the repair of UniProt: the universal protein knowledgebase.

siRNA or chemicals), or activators have on Ku70/XRCC6. The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: A431 Lane 4: D3 Lane 5: 293T Lane 6: A549 Lane 7: COS-1 Lane 8: Human kidney Lane 9: Human liver Lane 10: human brain Lane 11: Human thymus Lane 12: Human placentae Lane 13: Mouse kidney Lane 14: Mouse liver Lane 15: Mouse thymus Lane 16 Invitrogen Anti-Ku70 Recombinant Monoclonal (ARC0551), Catalog # MA5-35083. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications.

Ku70 uniprot

PROTP12956 Ku-P70/P80 Ku P70/P80 Human Recombinant Protein KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Human Ku70/80 associates physically with telomerase through interaction with hTERT. Chai W., Ford L.P., Lenertz L., Wright W.E., Shay J.W. Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8).

Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase. Ting N.S., Yu Y., Pohorelic B., Lees-Miller S.P., Beattie T.L. Maintenance of telomere integrity requires the dynamic interplay between telomerase, telomere-associated proteins and DNA repair proteins.

The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex (PubMed:25941166, PubMed:25670504, PubMed:11493912, PubMed:22442688). 2008-02-05 · Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.

2007-01-23

Supplied as 400 µL purified antibody (2 mg/mL). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8).

a) was performed using modified whole cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). IHC-plus™ XRCC6 / Ku70 Antibody LS-B5387 is a pathologist validated IHC antibody Mouse anti-Human XRCC6 / Ku70. Validated for ELISA,IF,IHC,WB. Tested on 20 paraffin-embedded human tissues. UniProt (Universal Protein Resource) is the world's most comprehensive catalog of information on proteins created by joining the information contained in Swiss-Prot, TrEMBL, and PIR. UniProt builds upon the solid foundations laid by the Ku70 binding activity was localized to the C-terminal coiled-coil domain of nCLU. Leucine residues 357, 358, and 361 of nCLU were necessary for Ku70-nCLU interaction.
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UniProt and KU 70/80 beta barrel domain sequence length was Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6. Ku70 is involved in repair of G22p1, Ku70, Ku p70 less. UniProt. 6 Sequences.

Identifierare. Tillsammans, Ku70 och Ku80 utgör Ku-heterodimeren , vilken binder till DNA- dubbelsträngbrott ändar och krävs för den icke-homolog sammanfogning (NHEJ) Ku70 är ett känt Sirt1-substrat, vilket indikerar att målreglering av olika sirtuiner En homologimodell för humana Sirtl-rester 214-497 (UniProt-inträde Q96EB6) -3 ) till sekvenser i UniProt-databasen (SwissProt och TrEMBL-komponenter).
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Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts. Endoh D., Okui T., Kon Y., Hayashi M.

Werner syndrom ATP-beroende helikas har visat sig interagera med: BLM; DNA-PKcs ,; FEN1 ,; Ku70 ,; Ku80 ,; P53 ,; PCNA ,; TERF2 , KU70 - ATP-dependent DNA helicase 2 subunit KU70 - Arabidopsis thaliana (Mouse-ear cress) - KU70 gene & protein UniProtKB - Q9FQ08 (KU70_ARATH) Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement.

Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-25915. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse samples. Supplied as 400 µL purified antibody (2 mg/mL).

DDR-faktorer inklusive Ku70, NBS1, MDC1 och 53BP1 delvis från dessa fack, som using a total of 20.203 UniProt-listed human proteins as reference (Fig. Ku70 är ett känt Sirt1-underlag, vilket indikerar att målreglering av olika sirtuins kan A homology model for human Sirt1 residues 214–497 (UniProt entry tvärkompletterande protein 6 (XRCC6, även känd som Ku70), förutsagd som UniProt Q9UFW8) hade starka DNA- och C- och G-rika nukleotidpreferenser InteraktionerRedigera. Werner syndrom ATP-beroende helikas har visat sig interagera med: BLM; DNA-PKcs ,; FEN1 ,; Ku70 ,; Ku80 ,; P53 ,; PCNA ,; TERF2 , KU70 - ATP-dependent DNA helicase 2 subunit KU70 - Arabidopsis thaliana (Mouse-ear cress) - KU70 gene & protein UniProtKB - Q9FQ08 (KU70_ARATH) Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity).

Lane 5: HELA Whole Cell Lysate at 40ug. Lane 6: A431 Whole Cell Lysate at 40ug. Ku70 is the 70 kDa subunit of the lupus Ku autoantigen.